Structure , Heterologous Expression , Family 19 Chitinases and Nam -
نویسنده
چکیده
We identified four new family 19 chitinases in Oi:yza sativa L. cv. Nipponbare: one class I (OsChiald), two clftss II (OsChia2a nd OsChia2b), and Qne class IV (OsChia4a). OsChia2a resembled (about 60% identity) the catalytic domains of class I chitinases, but OsChia2b was almost identical (95% identity) to tbat of the class IV enzyme. OsChialc, OsChialcACBD (a deJetion of OsChialc lacking a chitin-binding domain, CBD), and OsChia2b were separately expressed and purified in Pichia pasterts. OsChialc inhibited fungal growth significantly more than OsChialcACBD or OsChia2b. The activities of these enzymes on chitin polymers were similar, but they acted diftrently on Macetylchitooligosac-
منابع مشابه
Structure, heterologous expression, and properties of rice (Oryza sativa L.) family 19 chitinases.
We identified four new family 19 chitinases in Oryza sativa L. cv. Nipponbare: one class I (OsChia1d), two class II (OsChia2a and OsChia2b), and one class IV (OsChia4a). OsChia2a resembled (about 60% identity) the catalytic domains of class I chitinases, but OsChia2b was almost identical (95% identity) to that of the class IV enzyme. OsChia1c, OsChia1c deltaCBD (a deletion of OsChia1c lacking a...
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